Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 1 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000661163.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 1 L
TBE 10X Solution is a buffered solution commonly used in molecular biology and biochemistry. This 1-liter bottle contains a highly concentrated solution of Tris, borate, and EDTA (ethylene diamine tetraacetic acid), which is used as a running buffer for agarose gel electrophoresis. TBE helps maintain the pH and ionic strength of the electrophoresis buffer system, which is critical for the proper migration of nucleic acids. This 10X concentrated solution can be diluted to 1X for use in standard gel electrophoresis procedures. It is an essential reagent for DNA analysis, particularly in applications involving high-resolution separation of nucleic acid fragments.
Bioworld Bis-Tris Propane, 1 kg
A non-zwitterionic buffer that is useful in the pH 6.3-9.5 rangeSynonym: 1,3-Bis[tris(hydroxymethyl)methylamino]propane Appearance: SolidBoiling Point : 573.3 °CDensity : 1.326 g/cm3IUPAC_Name: 2,2'-(1,3-Propanediyldiimino)Bis[2-(Hydroxymethyl)-1,3-Propanediol]Melting Point: 164-165 °CPH Value: 6.3 - 9.5
Bioworld TTTRIS-TRICINE)-SDS BUFFE
TT (TRIS-TRICINE)-SDS BUFFER, READY PACK 10X: Tris-Tricine-SDS Buffer Tris-Tricine SDS (TT-SDS) Buffer has been developed for both non-denaturing and denaturing electrophoresis of proteins, and offers several advantages over more convent...
Pfanstiehl Inc Tris Base (Tromethamine) High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP (T-153) 5KG GMP, Multi-compendial CAS: 77-86-1 Injectable Excipient Grade
Pfanstiehl Tris Base, High Purity, Low Endotoxin, Low Metals, USP, EP, BP, ChP is a multi-compendial buffer component that has been specifically designed and formulated to remove impurities such as endotoxin, trace metals, and nitrosamines. Manufactured in the USA under ICH-Q7 cGMP conditions, Pfanstiehl Tris Base meets parenteral (injectable) excipient grade standards, making it ideal for pharmaceutical, biologic, and vaccine bioprocessing and formulation. Pfanstiehl Brand Tris Base is compliant with USP (United States Pharmacopoeia) EP (European Pharmacopoeia), BP (British Pharmacopeia) and ChP (Chinese Pharmacopeia) Compendial, U.S.P. , cGMP , pharmaceutical grade .
Chem-Impex International, Inc. Ethylenediaminetetraacetic acid | 60-00-4 | MFCD00003541 | 100G
Ethylenediaminetetraacetic acid, 60-00-4, MFCD00003541, 100G
Bioworld TBE Urea Running Buffer (10X), 4 L
Product DescriptionTBE(Tris-Borate-EDTA) urea running buffer is used for protein analysis. It has a 10X concentration and contains 0.89M Tris base, 0.89M boric acid, 20 mM EDTA, and a pH at 8.3.For laboratory use only. Not fit for agricultural, household, clinical use or human consumption.
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Buffer; National Diagnostics; Tris-Glycine-SDS PAGE (10X); Formulated w/18 Megohm water and 0.2u filtration; Saves time and improves results; Size: 1L
Bioworld Tris-Acetate-SDS Running Buffer (10X), 500 mL
TRIS-Acetate-SDS Running Buffer (10X) All bioWORLD running buffers are prepared with high quality reagents. Technical Information:Dilute with deionized water to make 1X. 10X concentration1X contains: 50mM Tris base, 50mM Tricine, 3.5mM SDS pH 8.24
Selleck Chemical LLC Trometamol hydrochloride S5300-100mg
Trometamol (THAM Tromethamine TRIS-HCL) hydrochloride a proton acceptor is a biologically inert amino alcohol of low toxicity which buffers carbon dioxide and acids in vitro and in vivo Trometamol rapidly restores pH and acid-base regulation in acidaemia
![Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000660682.jpg-250.jpg)
Alkali Scientific TBE 10X Solution [TRIS borate EDTA 10X Solution], 4 L
TBE 10X Solution is an essential buffer used in the electrophoresis of nucleic acids such as DNA and RNA. This 4-liter bottle contains a concentrated mixture of Tris, borate, and EDTA, which provides the necessary pH and ionic environment for the proper migration of nucleic acid samples. TBE is particularly effective for separating small DNA fragments and is widely used in both research and clinical laboratories. By diluting the 10X stock to 1X, laboratories can prepare a buffer suitable for use in standard electrophoresis procedures. It is an important reagent for molecular biology applications requiring high-purity separation of nucleic acids.
